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IsoScreen Procedures

  1. IsoScreen is supplied loosely assembled in two parts inside a sealed pouch. The upper syringe is for sample collection. The lower part is where the reaction or test takes place.

  1. IsoScreen detects the presence of isoniazid breakdown products.

  1. Unprocessed urine should be collected in a container with a wide neck, such as our urine collecting cup.

  1. Draw the urine into the syringe by holding the plunger of the syringe between the thumb and finger while supporting the cap with the other fingers. The plunger should be pulled to the end. There will be a small bubble of air in the syringe.

  1. Place the syringe inside the barrel. In which is a membrane positioned half-way down the tube. Place the tip of the syringe lightly onto the membrane.

  1. Holding the white cap and using the small flanges on the side push down evenly with two thumbs so forcing the syringe down and through the membrane. This is best done with a quick snap action.

  1. The 'click' will indicate the cap is now in place and the test activated.

  1. IMMEDIATELY push down the plunger, squirting the urine sample into the tube and on top of the chemicals.

  1. QUICKLY mix the sample and urine together. This is best done by holding the cap FIRMLY between finger and thumb of one hand and tapping the bottom of the tube repeatedly with the forefinger of the other hand. This will cause a vortex or eddy in the liquid. Alternatively, a vortex mixer can be used. Mix the liquid for 30 seconds.

  1. The tablet in the liquid will start to fix and bubble. If the urine sample is from a patient who has taken their isoniazid after about 1 minute the liquid will start to turn blue/black. Leave the test to develop the correct colour for 5 minutes after addition of the sample.

  1. A dark blue/black indicates treatment adherence, green means the tablet has been taken, but not for about two day and yellow means no treatment taken within the last few days.
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